Fig. 4

Analysis of DEmRNAs and DEncRNAs. (A) PCA correlation analysis. (B) Volcano plot of control group vs. model group. (C) Volcano plot of model group vs. ACU group. (D) DEmRNAs and DEncRNAs were validated by RT-qPCR (n = 3). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Note: In Figs. 4B-C, the two vertical dashed lines represent the threshold for fold change, while the horizontal dashed line represents the significance threshold. Red circles represent upregulated DEmRNA and DEncRNA, blue circles represent downregulated DEmRNA and DEncRNA, and gray circles represent mRNA and ncRNA with no statistically significant differences