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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis

Fig. 2

Effects of NEAT1 on proliferation, apoptosis, and cell cycle of KGN cells. (a) FISH assay was used to determine the subcellular localization of NEAT1 in KGN cells. (b) RT-qPCR was performed to detect the transfection efficiency of the OE-NEAT1 and si-NEAT1 plasmids. (c–d) CCK-8 and EdU assays were used to detect the effect of NEAT1 on the viability and proliferative capacity of KGN cells. (e–f) Flow cytometry was used to assess the effect of NEAT1 on apoptosis and cell cycle of KGN cells. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

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