Fig. 3

miR-204-5p is a target of lncRNA NEAT1. (a) Downstream targets of NEAT1 were predicted using the starBase, miRcode, and LncBase databases. (b) RT-qPCR was used to detect the expression levels of miR-125a-5p and miR-204-5p in human GCs. (c) Bioinformatics software was used to predict the binding sites of NEAT1 and miR-204-5p. (d) A dual-luciferase reporter assay was used to verify the target-binding relationship between NEAT1 and miR-204-5p. (e) RT-qPCR was used to detect miR-204-5p expression levels following NEAT1 overexpression or silencing. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001