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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis

Fig. 4

Effects of miR-204-5p on proliferation, apoptosis, and cell cycle of KGN cells. (a) RT-qPCR was used to analyze miR-204-5p expression levels following transfection with mimics-miR-204-5p or inhibitor-miR-204-5p. (b–c) CCK-8 and EdU assays were performed to detect the effects of miR-204-5p on KGN cell viability and proliferation. (d–e) Flow cytometry was performed to assess the effects of miR-204-5p on KGN cell apoptosis and cell cycle. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

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