Fig. 7
Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were assessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expression levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) ELISA was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001