Fig. 8
NEAT1 regulates steroid hormone biosynthesis and the MAPK signaling pathway in KGN cells via the miR-204-5p/ESR1 axis. (a-b) RT-qPCR and western blotting were performed to evaluate the effect of NEAT1 on ESR1 mRNA and protein expression levels, respectively. (c) RT-qPCR was performed to analyze the expression of key genes involved in steroid hormone synthesis. (d) ELISA was performed to determine E2 levels. (e) western blotting was performed to determine the levels of proteins related to steroid hormone synthesis and MAPK signaling pathway. (f-g) RT-qPCR and western blotting were used to assess the expression levels of steroid synthase and key molecules of the MAPK pathway in human GCs, respectively. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001