LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis

Table 1 Primer sequences for gene expression

Gene	Primer sequence (5’-3’)
NEAT1	Forward: AGTGTGAGTCCTAGCATTG
NEAT1	Reverse: GAACTTCCTCCTCCTAAGC
hsa-miR-204-5p	Forward: CGCGTTCCCTTTGTCATCCT
hsa-miR-204-5p	Reverse: AGTGCAGGGTCCGAGGTATT
hsa-miR-125a-5p	Forward: TCGGCAGGTCCCTGAGACCCTT
hsa-miR-125a-5p	Reverse: CTCAACTGGTGTCGTGGA
StAR	Forward: GCCACATTTGCCAGGAAACAATG
StAR	Reverse: CTCCTGGTCACTGTAGAGAGTCT
CYP19A1	Forward: GCAAAGCACCCTAATGTTGAAGA
CYP19A1	Reverse: CGAGTCTGTGCATCCTTCCAATA
ESR1	Forward: CTCTAACCTCGGGCTGTGC
ESR1	Reverse: AGATGCTTTGGTGTGGAGGG
NOTCH2	Forward: ATCCCACAAAGCCTAGCACC
NOTCH2	Reverse: CCTTGTCCCTGAGCAACCAT
IGFBP5	Forward: GAAGCAGATGTGTCTCTGCCC
IGFBP5	Reverse: TTCCCTCTGCTTCCTGGTTC
GAPDH	Forward: TGCACCACCAACTGCTTAGC
GAPDH	Reverse: GGCATGGACTGTGGTCATGAG
U6	Forward: CAGCACATATACTAAAATTGGAACG
U6	Reverse: ACGAATTTGCGTGTCATCC

CYP19A1, cytochrome P450 family 19 subfamily A member 1; ESR1, estrogen receptor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IGFBP5, insulin-like growth factor binding protein 5; NEAT1, nucleo-enriched abundant transcript 1; NOTCH2, recombinant notch homolog 2; StAR, steroidogenic acute regulatory protein; U6, RNU6

ISSN: 1757-2215